CATALOG INDEX
BY ALPHABETICAL
ORDER

CATALOG INDEX
BY DEPARTMENT

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- [ Indy Air Thermocycler ]
- [ RapidCycler ]

RapidCycler

Engineered to match the speed of biochemistry Combining the ideal sample container with the ultimate heating and cooling medium — high velocity air The standard for more than 5 years 10° per/sec Peak Rate High Temperature DNA Amplification 30 Cycles in 8 minutes 48 Sample Capacity User Friendly Interface Maximum Run 1,536 Samples per Day 99 Cycle Programs

"The RapidCycler can complete a 30 cycle reaction in less than 10 minutes. Finally, a machine that can keep up with the speed of the biochemistry."

Efficiency
Heat block cyclers can take hours to do 30, three temperature cycles. This slow rate leads to long lines for the machine and sign up sheets that force you to start your reaction at 2 a.m.. The RapidCycler™ can complete a 30 cycle reaction in less than 10 minutes. Finally, a machine that can keep up with the speed of the biochemistry.

Why are other thermocyclers so slow? Thermocyclers generally use microcentrifuge tubes or modified microtiter plates. These plastic containers insulate the sample. The temperature of a 100 µl sample within a microcentrifuge tube lags 20-40 secs behind the block temperature. This, combined with sluggish temperature ramp rates of heating blocks, explains why they are so slow.

There is another major factor - temperature hold times. A common protocol for temperature cycling is 1 min at 94°C, 2 min at 55°C and 3 min at 72°C. Even if instantaneous temperature transitions were possible, one cycle would take 6 minutes. The actual enzymatic extension occurs in about 10 seconds for a 500 bp fragment. This leaves 97% of the total time wasted, not even counting ramp time. What are these machines doing? They are waiting for steady-state conditions to bring all the samples to roughly the same temperature. Unfortunately, because of the geometry of the heating elements and metal blocks, even the steady-state condition may not produce uniform temperatures across all samples. At best, these machines suffer from temperature imbalance during the long wait for equilibrium conditions. At worst, the imbalance is permanent.

Savings
The high sample throughput of the RapidCycler allows more users per machine.

• Optimized for 10µl reaction volumes saving you on reagent costs.
• 25 and 50µl capillaries and thin walled microcentrifuge tubes also provide excellent results.

Maximum Yield
As a reaction proceeds, the efficiency decreases. This is due in part to competition between primers and reaction product. Rapid cool-down (>5°C/sec) favors the kinetics of primer annealing over the thermodynamic advantage of product reannealing. This in turn yields a higher final concentration of product.

Cleaner Product
Rapid cycling leaves less time for primer extension at non-specific annealing sites. The amount of nonspecific product is directly related to the time spent at low temperatures.

• Cleaner electrophoresis gels
• More readable gels
• Programmable hold times
• Maximum specificity