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 Specifications
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The new SPECTRAmax® PLUS384 is the only spectrophotometer with a built-in cuvette port and microplate drawer. Now you can run both standard spectrophotometer and microplate reader applications on the same instrument. Read one sample or up to 384 at a time – it’s your choice. Use any standard cuvette, 12 x 75 mm test tube, 96- or 384-well microplate. For even more sample throughput, the SPECTRAmax PLUS384 can be easily integrated into a full robotic system. The SPECTRAmax PLUS384 easily adapts to fit your sample processing requirements.
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Patented PathCheck® Sensor
PathCheck Sensor eliminates the last major difference between microplate readers and spectrophotometers - optical pathlength. PathCheck Sensor provides a powerful new tool for microplate analysis.
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Until now, you could never get the same absorbance readings from a microplate reader as from a spectrophotometer because samples in a microplate have a volume-dependent pathlength. PathCheck Sensor is the only patented technology available that measures the depth (optical pathlength) of samples in the microplate. SOFTmax® PRO software can automatically normalize the well absorbance to a cuvette equivalent pathlength of 1 cm. It's like having 96 or 384 cuvettes! |
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Eliminate standard curves. For compounds with a known extinction coefficient, concentration can be calculated directly from absorbance. |
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Pipetting does not have to be accurate. With the PathCheck Sensor, it is acceptable to have different volumes in the wells. Just put 20 µl - 300 µl of sample or standard into each well and read the plate. The PathCheck Sensor will correct for the volume differences in all 96 or 384 wells, and report 1 cm absorbance values. |
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Extend the dynamic range to 6+ OD. If a well reads out of range (> 4 OD), decrease the volume (100 µl of sample can be used with excellent precision) in that well and reread the plate using the PathCheck Sensor. A 100 µl sample (optical pathlength ~0.3 cm) that reads 2.8 OD will be corrected to ~9.2 OD. |
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Detect pipetting errors in 96- or 384-well microplates. The PathCheck Sensor measures differences in volume between the wells, so you can identify and eliminate sources of error from your data or correct your absorbance values. |
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Quickly and easily test multichannel dispensers and pipettors. By measuring the depth of the liquid in the well, you can determine the volume of liquid dispensed. Even 96- or 384-channel dispensers can be tested in a matter of minutes. No need for dyes. Detect volumes as low as 100 µl in 96-well standard microplates, 30 µl in half-area or 384-well microplates, and 4 µl using a liquid addition method in half-area well microplates. |
Applications
Essentially, all UV/VIS spectrophotometric assays that are read in a conventional spectrophotometer or microplate reader can be read in the SPECTRAmax PLUS384:
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Colorimetric assays |
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ELISAs/EIAs |
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Immunoassays |
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Drug dissolution profiles |
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Enzyme kinetics (e.g., Ki, Km, etc.) |
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Kinetic turbidimetric methods |
The Best Performance by Design
The SPECTRAmax optical system is the only multichannel design that truly mimics a dual-beam spectrophotometer. Each sample has a discrete sample beam and reference beam so that each well is measured directly, eliminating error due to variations in light output between the fibers. The 9 channel system (8 for the microplate and 1 for the cuvette port), consisting of 9 sample beams and detectors and 9 reference beams and detectors, delivers both superior precision and speed of reading across the microplate through 4.0 OD.
The SPECTRAmax Optical System
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SOFTmax® PRO for Windows® 95/98/2000/NT or Mac™ OS*
SOFTmax PRO, the industry-leading microplate reader
software for the SPECTRAmax PLUS384 system allows you to completely
customize your data collection and analysis. Choose endpoint, kinetic,
or spectral scans; view kinetic and spectral runs in real time; and collect
and store data from multiple plates, cuvettes, graphs, analysis tables,
and notes sections in the same data file. You can automate lab processes
with SOFTmax PRO software's interprocess messaging features, and export
and import data and template information to and from your LIMS system.
In addition, a complete validation package is available for SOFTmax PRO.
*For version version compatiblity information for using Mac OS with SOFTmax PRO, please see the SOFTmax PRO page.
| Streamline GLP/GMP Compliance Optical Performance: the SPECTRAtest™ Validation Package provides a complete NIST-traceable solution for validating optical performance of the SPECTRAmax PLUS384 and does it automatically. All 9 test measurements and calculations are handled by SOFTmax PRO software and summarized in one report. A NIST-traceable cuvette set is also available to validate the cuvette port. SPECTRAtest makes it easy for you to be GLP/GM-compliant. Software Validation |
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Specifications**
| Photometric Performance | ||
| Wavelength Range | 190 - 1000 nm | |
| Wavelength Selection | Monochromator, tunable 1.0 nm increments | |
| Wavelength Bandwidth | 2 nm | |
| Wavelength Accuracy | < ± 1.0 nm | |
| Wavelength Repeatability | ± 0.2 nm | |
| Photometric Range | 0.0 to 4.0 OD | |
| Photometric Resolution | 0.001 OD | |
| Photometric Accuracy (Microplate) | < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD | |
| Photometric Accuracy (Cuvette) | < ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD | |
| Photometric Precision | < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD | |
| Photometric Baseline Flatness | < 0.001 OD | |
| Stray Light | < 0.05% @ 230 nm | |
| Light Source | Xenon flash lamp | |
| Microplate Read Time | Normal Mode 96 wells. . . . 9 seconds 384 wells. . . .29 seconds Speed Read Mode |
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| Temperature Regulation Specifications | ||
| Temperature Range | Ambient + 4 C up to 45 C | |
| Temperature Uniformity (Microplate) | ± 0.5 C @ 37 C | |
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| Physical Specifications | ||
| Dimensions | 22 cm (h) x 58 cm (w) x 38 cm (d) | |
| Weight | 30 lb (13.6 kg) | |
| *U.S. Patent Number 5,959,738 **Specifications subject to change. |
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