E*Colite
- Presence/Absence of coliforms and E.coli in water
- Unique single use, self contained Burst-a-Seal bag
- Thiosulfate and bactericide included
- Coliform results in 28 hours
- Easy interpretation of blue color development
E*Colite is a carbohydrate rich, selective medium particularly
adept at resuscitation and detection of chlorine stressed coliform and
E.coli encountered in drinking, bottled and food/beverage waters.
Simple — The bag design houses all test components. Simply
add water sample no preparation, no test bottles. The bag also acts as a
sample container. There are no collection bottles or loose sodium
thiosulfate tablets. Reagents are stable up to 12 months at room
temperature.
Procedure — Add water sample and seal the bag. Push the
water into the medium compartment. Incubate at 35°C for 28 hours.
Interpretation — If the sample turns blue, coliforms are
present. If a blue coliform positive sample fluoresces, E.coli are
present. If a blue sample does not fluoresce, continue incubating an
additional 20 hours (total 48 hours). If blue growth fluoresces after the
48 hours, the sample is confirmed E.coli. If the blue bag does not
fluoresce after 48 hours, the sample is coliform positive and E.coli
negative.
Easy- E*Colite uses X-gal indicator to produce an indigo blue
positive reaction. This blue color, as opposed to traditional yellow
color, vastly improves interpretation of turbid or rust and heterotropic
bacteria contaminated waters. All reagents and containers are self
enclosed, reducing space for supplies and eliminating accessory inventory,
tracking and storage.
Sensitive- E*Colite detects a single microorganism in 100ml
water and has been demonstrated to have 98% correlation with the LTB/BGLB
method in detecting 4 log chlorine stressed coliform. It demonstrates a
93% correlation with LTB/ECMUG in detecting 4 log chlorine stressed
E.coli.
Specific- E*Colite uses selective detergents to minimize
non-specific bacteria interferences. It recovers stressed organisms more
effectively than LTB/BGLB or ECMUG (reference method). In a 500 sample
comparability study with 4 log chlorine stressed coliform, E*Colite
recovered 53% positive coliform while reference recovered 48%. Stressed
E.coli were recovered 57% in E*Colite versus 48% by reference.
Bio-safe- A self enclosed bactericide is provided to
introduce a 7-8 log reduction of any grown organisms. The bactericide
provides critical bio-safety for operations without access to an
autoclave.
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