SPECTRAmax 190 Microplate Spectrophotometer

The SPECTRAmax 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. For aqueous solutions, our patented* PathCheck^® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The corrected absorbance is within 5% of the value obtained in a conventional spectrophotometer.

The SPECTRAmax 190 microplate reader allows you to:

use the extinction coefficient of your sample to calculate concentrations directly

correct for volume differences in the wells

expand the dynamic range of your assay

detect pipetting errors

check performance of your pipetting devices

Spectral Scanning Makes Assay Development Easy
You can select the optimum wavelength for reading your assay by viewing the complete spectrum of your sample, allowing you tomonitor the stability of your sample or reagent, determine interfering compounds or conditions of your assay, and validate your assay to regulatory standards.

Accurate Nucleic Acid Measurements
The SPECTRAmax® 190 microplate spectrophotometer is the tool you need for DNA analysis. A bandwidth of 5 nm or less is required for accurate absorbance measurement of DNA¹. With the ability to detect DNA down to 25 ng per well, as well as a limit of calculation to 75 ng per well, the SPECTRAmax 190 microplate spectrophotometer gives you the same sensitivity as a conventional spectrophotometer.

Expand the Dynamic Range of Any Assay to 0 to 6+ OD
The PathCheck sensor can extend the instrument linearity by a factor of three or more. For very concentrated samples, use a minimum volume in the microplate well (100 µL has a pathlength of approximately 0.30 cm), then use the PathCheck sensor to calculate an equivalent 1-cm pathlength absorbance value, giving you calculated absorbance values well beyond the capabilities of other instruments.

Quickly Determine Multi-Channel Pipettor Performance
Precision and accuracy of multi-channel pipettors can now be verified using the actual dispense reagent without the need to add a dye. By measuring the depth of the liquid in the well, the patented PathCheck sensor can determine the volume of the liquid dispensed². Even 96-channel dispensers can be tested in a matter of minutes.

Specifications*

Photometric Performance
Wavelength Range		190 - 850 nm
Wavelength Selection		Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth		2 nm
Wavelength Accuracy		<± 2.0 nm
Wavelength Repeatability		± 0.2 nm
Photometric Range		-0.3 to 4.0 OD
Photometric Resolution		0.001 OD
Photometric Linearity (405 nm)		0 to 3.000 OD
Photometric Accuracy (190-850 nm)		< ±0.006 OD ±1.0%, 0 - 2.0 OD
Photometric Precision (190-850 nm)		< ±0.003 OD ±1.0%, 0 - 2.0 OD
PathCheck Measurement Error		< 5% when compared to cuvette reading
Stray Light		< 0.05% @ 230 nm
Light Source		Xenon flash lamp
Microplate Read Time
	Endpoint	12 seconds
	Kinetics	9 second minimum interval

Temperature Regulation Specifications
Temperature Range		Ambient + 4 C up to 45 C
Temperature Uniformity (Microplate)		± 0.5 C @ 37C, well-to-well
Temperature Stabilization Time		30 minutes maximum upon initiation
Reading Chambers		Isothermal when temperature regulation not enabled, <1 C

Physical Specifications
Dimensions		22 cm (h) x 58 cm (w) x 38 cm (d)
Weight		30 lb (13.6 kg)
*Specifications subject to change.
1 To measure absorbance accurately, the ratio of the instrument bandwidth to the sample bandwidth must be ~10% or ~5 nm for DNA. (Knowles, A.; Burgess, C. (Eds.): Practical Absorption Spectrometry; Chapman and Hall, London (1984)) *U.S. Patent Number 5,959,738


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